During the microinjection process, the collected eggs are incubated in incubators containing 37 degrees and 6% CO2 for 2-3 hours in culture solutions to complete their maturation. During this time, a semen sample is prepared. After 2-3 hours, the cells around the eggs are cleaned and their maturity is checked. Only mature eggs should be processed. After the eggs are cleaned, the microinjection process is performed.

Embryo Quality

The quality of the embryos is evaluated according to some shape (morphological) parameters after fertilization. The morphology of the embryos is evaluated under a microscope and according to the following criteria.

• Embryo cell (blastomere) count
• Embryo blastomere size (equal or unequal blastomeres)
• Presence and percentage of fragmentation (non-viable cell debris) In the light of all these criteria, embryos are classified according to their quality (grade) as follows:
• Grade I embryos:  These embryos have equal-sized, regular blastomeres and no fragments.
• Grade II embryo:  Contains blastomeres of equal size but
  contains a small amount of fragments (<20%).
• Grade III embryo:  Blastomeres are unequal and fragments are abundant.
  (20%-50%)
• Grade IV embryo:  Blastomeres are unequal and fragments are more than 50%. Studies have shown that the chance of pregnancy is higher with the transfer of Grade I and Grade II embryos than with the transfer of Grade III and Grade IV embryos due to the higher developmental potential of these embryos.